MICRONAUT Special Plates

Antimicrobial susceptibility testing with standard MIC plates covering an extended spectrum of organisms in microbiology

Test Principle

The antimicrobial susceptibility testing of the MICRONAUT-S/-AM plates is based on the rehydration of antibacterial agents and/or antifungal agents by the addition of a standardized suspension of bacteria or yeasts. The plates can be read after an incubation of 24-48 hours at a temperature of 30°C or 35-37°C (depending on test system and organism) with a reader or visually. Data recorded by automated reading can be evaluated and interpreted by MICRONAUT software.

PRODUCTS

MICRONAUT-S MRSA GP

Microtitration plates for the automated or manual susceptibility testing of multi-resistent Staphylococci, Enterococci and Pneumococci

Principle, shelf-life and storage

The susceptibility testing is based on the rehydration of antibiotics by adding a standar­dized bacteria suspension (Mueller-Hinton II broth). The result is measured photometri­cally after 18-24 hours incubation at 35-37°C. Results are going to be measured and interpreted either with the MICRONAUT software or visually. Due to a special vacuum drying method the plates can be stored at a room temperature of 15-25°C. The MICRONAUT test plates have a shelf life of 24 months at date of production.

Antibiotics configuration

The antibiotics configuration of the MICRONAUT-S MRSA GP plate allows the specific detection of the clinically relevant single or multi-resistances of gram-positive bacteria of nosocomial infection. The susceptibility testing with highly effictive reserve antibiotics like the MRSA cephalosprine Ceftaroline offers alternatives in case of extreme multi-resistance.

Procedure

  • Produce bacteria suspension in NaCl (McFarland 0.5)
  • Transfer in Mueller-Hinton II broth
  • Inoculate MICRONAUT-S test plate
  • Incubate for 18-24 hours at 35-37°C
  • Measure photometrically and interprete with MICRONAUT software

Susceptibility testing of staphylococci

  • Penicillin G MIC: detection of staphylococcal penicillinases
  • Detection of Oxacillin resistance by MIC determination with Oxacillin and Cefoxitin
  • Detection of Oxacillin borderline resistance by detection of Cefoxitin susceptibility
  • Detection of the induced MLSB resistence by Erythromycin/Clindamycin combination test according to CLSI standard
  • Testing of highly effective antibiotics like Vancomycin, Linezolid, Tigecycline, Daptomycin, and Ceftaroline as therapeutic options in case of extreme multi-resistance
  • Test interpretation according to EUCAST and CLSI standard

Susceptibility testing of enterococci

  • Ampicillin MIC: detection of ampicillin resistance
  • Detection of the phenotypical glycopeptide resistance pattern of Vancomycin resistant enterococci by determination of the MIC via Teicoplanin and Vancomycin
  • Differentiation between Enterococcus faecium and Enterococcus faecalis by determination of the MIC via Synercid®
  • Detection of HLAR strains through high-level-resistance testing via Gentamicin and Streptomycin
  • Testing of highly effective antibiotics as therapeutic options in case of extreme multi-resistance
  • Test interpretation according to EUCAST and CLSI standard

Susceptibility testing of pneumococci

  • Penicillin G MIC: detection of PBP changes
  • Detection of Cefotaxim resistance
  • Detection of Erythromycin resistance
  • Detection of Vancomycin resistance
  • Moxifloxacin MIC: detection of group IV quinolones resistance
  • Test of highly effective antibiotics as therapeutic options in case of extreme multi-resistance
  • Test interpretation according to EUCAST and CLSI standard

The details about the antibiotics coatings can be found here: Product description MICRONAUT-S MRSA GP

MICRONAUT-S ß-Lactamases (MBL, ESBL, KPC, AMP-C, OXA-48)

Phenotypical detection of multiple ß-Lactamases (multiple resistance determinants) in a single test system

Based on the micro-dilution procedure MICRONAUT‑S provides the phenotypical detection of clinically relevant cephalosporinases and carbapenemases at entero-bacteria and non-fermenters.

NEW: Extended spectrum of antibiotics by Temocillin and Ertapenem-/Meropenem screening concentrations for detection of OXA-48 like Type D-carbapenemases

In recent years there was an increase worldwide in carbapenem resistance based on type D-carbapenemase producing Enterobacteriaceae (type OXA-48). These secondary, plasmid encoded type D-carbapenemases can be spread horizontally by plasmid transfer and therefore showing a potential of dissemination of high epidemiologic importance. Moreover the ß-Lactamases resistance determinants are localized on mobile genetic elements (integrons) which are normally associated with additional resistance genes (e. g., aminoglycosides, fluoroquinolones etc.) bearing the high risk for the spread of multi-resistance among clinical relevant pathogens.

The novel standard plate MICRONAUT-S ß-Lactamases now includes temocillin and ertapenem-/meropenem screening concentrations for detection of low-level carbapenem resistance. These modifications improve the sensitivity of the test system for detection of OXA-48 like type D-carbapenemases.

Extended concentration range for ESBL and AMP-C detection

In the new MICRONAUT-S ß-Lactamases plate the drug concentrations of cefepime, cefotaxime, and ceftazidime are increased up to 128 µg/ml. Based on the new drug concentration ranges the sensitivity of the ESBL- and AMP-C confirmatory assay is raised by the reduction of “out of range” results.

An overview about the scope of the enhanced plate MICRONAUT-S ß-Lactamases

  • Resistance determination of all relevant gram-negative bacteria (enterobacteria, Aeromonas, non-fermenters) against 6 antibiotics and 7 antibiotics combinations in a standard micro-dilution procedure
  • Phenotypical detection of MBL (Metallo- ß-Lactamases) by resistance determination against Meropenem as a mono substance and in combination with the divalent cationic chelator EDTA
  • Phenotypical detection of ESBL (Extended Spectrum ß-Lactamases) by the susceptibility testing with 3 extended spectrum cephalosporins and their combination with Clavulan acid
  • Phenotypical detection of KPC (Klebsiella pneumoniae carbapenemase) by resistance determination against Meropenem as a mono substance and in combination with 3- Amino-Phenyl-Borat (3- APB)
  • Phenotypical detection of AMP-C (Aminopenicillin inactivating cephalosporins) by the susceptibility testing with 3 extended spectrum cephalosporins and their combination with 3- Amino-Phenyl-Borat (3- APB)
  • Detection of OXA-48 like type D-carbapenemases based on the determination of temocillin high-level resistance
  • Detection of low-level carbapenem resistance by introduction of ertapenem-/meropenem screening concentrations
  • Analysis and interpretation after visual or automated reading
  • MICRONAUT software for the analysis and interpretation after automated reading with:
  1. Indication of confirmed resistance mechanisms (ESBL | MBL | KPC | AMP-C | OXA-48 like) by MIC quotient assessment and determination of temocillin high-level resistance
  2. Phenotypcal confirmation of resistance mechanisms even at the occurrence of multiple ß-Lactamases

The detailed layout is described here: Product description MICRONAUT-S ß-Lactamases

MICRONAUT-S MDR MRGN-Screening

MIC plate for the susceptibility testing of multi-resistent gram-negative bacteria

Based on the micro-dilution procedure MICRONAUT-S MDR MRGN Screening provides the phenotypical detection of clinically relevant cephalosporinases and carbapenemases at entero-bacteria and non-fermenters.

NEW ⇒  Extended spectrum of antibiotics by meropenem screening concentrations for an improved detection of OXA-48 like Type D-carbapenemases

NEW ⇒  Extended spectrum of antibiotics by introduction of the novel antibiotic combinations ceftazidime-avibactam and ceftolozane-tazobactam

  • Resistance determination of all relevant gram-negative bacteria (enterobacteria, Aeromonas, non-fermenters) against antibiotics and antibiotics combinations in a standard micro-dilution procedure
  • Antimicrobial susceptibility testing against the novel antibiotic combinations ceftazidime-avibactam and ceftolozane-tazobactam
  • Phenotypical detection of MBL (Metallo- ß-Lactamases) by resistance determination against Meropenem as a mono substance and in combination with the divalent cationic chelator EDTA
  • Phenotypical detection of KPC (Klebsiella pneumoniae carbapenemase) by resistance determination against Meropenem as a mono substance and in combination with 3- Amino-Phenyl-Borat (3- APB)
  • Phenotypical detection of AMP-C (Aminopenicillin inactivating cephalosporins) by the susceptibility testing with 3 extended spectrum cephalosporins and their combination with 3- Amino-Phenyl-Borat (3- APB)
  • Detection of OXA-48 like type D-carbapenemases based on the determination of temocillin high-level resistance
  • Detection of low-level carbapenem resistance by introduction of meropenem screening concentrations
  • Analysis and interpretation after visual or automated reading according to EUCAST or CLSI standard
  • MICRONAUT software for the analysis and interpretation after automated reading with:

  1. Indication of confirmed resistance mechanisms (MBL | KPC | AMP-C | OXA-48 like) by MIC quotient assessment and determination of temocillin high-level resistance
  2. Phenotypcal confirmation of resistance mechanisms even at the occurrence of multiple ß-Lactamases

MICRONAUT-S Pneumococcus and Haemophilus

  • For detection of resistence by MIC determination towards relevant antimicrobial agents

MICRONAUT-S Anaerobs MIC

  • For MIC determination towards antimicrobial agents with high avctivity against anaerobes inclusive compounds like tigecycline, moxifloxacin, or ertapenem

MICRONAUT-S Campylobacter

  • For detection of resistence by MIC determination towards all Campylobacter-relevant antimicrobial agents

MICRONAUT-AM MHK

MIC plates for the automated or manual susceptibility testing of yeast

The susceptibility testing is based on the rehydration of antimycotics by adding a standardized yeast suspension. After incubation of 24-48 hours at 35-37ºC the plates are ready to be measured photometrically or be interpreted visually. Because of a special vacuum drying process the plates can be stored at a room temperature of 15-25°C. MICRONAUT test plates have a shelf life of 24 months at date of production.

Features of the MICRONAUT-AM MHK plate

  • Susceptibility testing of 9 antimycotics in up to 11 concentrations following the MIC procedure (minimum inhibitory concentration)
  • Contains the antimycotics Anidulafungin, Caspofungin and Posaconazol
  • The included negative control simplifies the visual as well as the photometrical reading
  • RPMI medium improves the growth of the yeast
  • Interpretation after 24 hours for yeast and 48 hours for cryptococci

Procedure

  • Preparation of yeast suspension in NaCl 0.9 % (McFarland 0.5)
  • Transfer to RPMI medium plus AST indicator
  • Inoculation of MICRONAUT-AM MHK test plate
  • Incubation at 35-37°C for 24-48 hours
  • Incubation period depending on color change (pink) of the growth control
  • Photometrical or visual reading of the test plates

MICRONAUT-AM KH

Microtitration plates for automated or manual susceptibility testing of yeast

The susceptibility testing is based on the rehydration of antimycotics by adding a standardized yeast suspension. After incubation of 22-24 hours at 35-37°C the plates are ready to be measured photometrically or interpreted visually. Because of a special vacuum drying process the plates can be stored at a room temperature of 15-25°C. MICRONAUT test plates have a shelf life of 24 months at date of production.

Features of MICRONAUT-AM KH plates

  • Susceptibility testing of 6 antimycotics in up to 8 concentrations following the MIC procedure (minimum inhibitory concentration)
  • Contains the modern antimycotics Caspofungin and Posaconazol
  • The included negative control simplifies the visual as well as the photometrical reading
  • RPMI medium improves the growth of the yeast
  • Interpretation after 24 hours for yeast and 48 hours for Cryptococci

Procedure

  • Preparation of yeast suspension in NaCl 0.9 % (McFarland 0.5)
  • Transfer to RPMI medium plus indicator
  • Inoculation of MICRONAUT-AM KH test plate
  • Incubation of the test plates at 35-37°C for 24-48 hours depending on color change (pink) of the growth control
  • Photometrical or visual reading of the test plates

Further MICRONAUT plates on demand. Don't hesitate to contact us. We develop the layout for your requirements.

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